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葡萄籽原花青素对胆汁酸盐在结肠腺细胞Caco-2中转运的影响
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篇名: 葡萄籽原花青素对胆汁酸盐在结肠腺细胞Caco-2中转运的影响
TITLE:
摘要: 目的:研究葡萄籽原花青素(GSP)对甘氨胆酸钠(GA)和牛磺胆酸钠(TA)在结肠腺细胞Caco-2中跨膜转运的影响。方法:利用Caco-2细胞模型,采用反相-高效液相色谱法测定细胞培养液中GA和TA的含量。试验分为GSP组、GA组、TA组、GSP+GA组和GSP+TA组,分别检测0、2、4、8 h从Transwell小室顶端(AP)侧透过Caco-2细胞向基底端(BL)侧转运GA和TA的透过量。结果:GA和TA检测浓度的线性范围均为0.05~1.2 mmol/L(R2=0.999 9)。随着时间的延长,GA组和TA组BL侧GA和TA的透过量急剧增加;而加入GSP后,BL侧GA和TA的透过量明显较少,差异有统计学意义(P<0.01)。结论:GSP对GA和TA在Caco-2细胞中的跨膜转运具有抑制作用。
ABSTRACT: OBJECTIVE: To study the effects of grape seed proanthocyanidin (GSP) on the transmembrane transport of sodium glycocholate (GA) and sodium taurocholate (TA) in colon glandular cell Caco-2. METHODS: Caco-2 model was used, and RP-HPLC was conducted to determine the contents of GA and TA in cell culture medium. The test was divided into GSP group, GA group, TA group, GSP+GA group and GSP+TA group, the transport volumes of transporting GA and TA from Transwell apical (AP) side to basolateral (BL) side by Caco-2 cell at 0, 2, 4, 8 h were detected, respectively. RESULTS: The linear ranges of GA and TA were 0.05-1.2 mmol/L (R2=0.999 9). With the time passing, transport volumes of GA and TA in BL site in GA group and TA group were sharply increased; while the transport volumes were obviously decreased after adding GSP, with statistical significance (P<0.01). CONCLUSIONS: GSP has inhibitory effect on the transmembrane transport of GA and TA in Caco-2 cell.
期刊: 2017年第28卷第10期
编辑: 来丽华,葛建
AUTHORS: LAI Lihua,GE Jian
关键字: 葡萄籽原花青素;结肠腺细胞Caco-2;牛磺胆酸钠;甘氨胆酸钠;跨膜转运
KEYWORDS: Grape seed proanthocyanidin; Colon glandular cell caco-2; Sodium glycocholate; Sodium taurocholate; Transmembrane transportation
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