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化浊解毒愈溃方对溃疡性结肠炎大鼠黏膜损伤的修复作用
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篇名: 化浊解毒愈溃方对溃疡性结肠炎大鼠黏膜损伤的修复作用
TITLE:
摘要: 目的:探讨化浊解毒愈溃方对溃疡性结肠炎(UC)大鼠黏膜损伤的修复作用及机制。方法:将72只大鼠随机分为空白组、模型组、美沙拉嗪组(阳性对照,0.3 g/kg)和化浊解毒愈溃方低、中、高剂量组(5、10、20 g/kg),各12只。除空白组外,其余各组大鼠均复制UC模型。造模4 d后,各给药组大鼠ig相应药物,空白组和模型组大鼠ig生理盐水,每天1次,连续2周。给药结束后,观察大鼠疾病活动指数(DAI)与黏膜损伤指数(CMDI);检测血清中白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)含量及结肠组织中环氧合酶2(COX-2)表达情况。结果:与空白组比较,模型组大鼠DAI和CMDI评分升高,血清中IL-8、TNF-α含量增加,结肠组织中COX-2表达增强,差异均有统计学意义(P<0.05)。与模型组比较,各给药组大鼠DAI和CMDI评分降低,血清中IL-8、TNF-α含量减少,结肠组织中COX-2表达减弱,差异均有统计学意义(P<0.05);与美沙拉嗪组比较,化浊解毒愈溃方中、高剂量组大鼠上述指标变化更为明显(P<0.05),且化浊解毒愈溃方的作用呈明显的量效关系。结论:化浊解毒愈溃方可改善UC大鼠的疾病活动状态、减轻结肠黏膜损伤,且中、高剂量作用优于美沙拉嗪。其作用机制可能与减轻炎症反应、抑制结肠组织中COX-2表达有关。
ABSTRACT: OBJECTIVE: To explore the repairing effect and mechanism of Huazhuo jiedu yukui formula on the mucosal damage of rats with ulcerative colitis (UC). METHODS: 72 rats were randomly divided into blank group, model group, mesalamine group (positive control, 0.3 g/kg), Huazhuo jiedu yukui formula low-dose, medium-dose, high-dose groups (5, 10, 20 g/kg), 12 in each group. Except for blank group, other groups were induced UC model. After 4 d of modeling, rats in administration groups received related medicines, ig, blank group and model group received normal saline, ig, once a day, for 2 weeks. After administration, disease activity index (DAI) and mucosal damage index (CMDI) of rats were observed; IL-8, TNF-α contents in serum and cyclooxygenase 2 (COX-2) expression in colon tissue were detected. RESULTS: Compared with blank group, DAI and CMDI scores in model group were increased; IL-8, TNF-α contents in serum were increased; COX-2 expression in colon tissue was enhanced, with statistical significances (P<0.05). Compared with model group, DAI and CMDI scores in model group were decreased; IL-8, TNF-α contents in serum were reduced; COX-2 expression in colon tissue was weakened, with statistical significances (P<0.05). Compared with mesalamine group, the above-mentioned indicators in Huazhuo jiedu yukui formula medium-dose, high-dose groups changed more obviously (P<0.05), and effects of Huazhuo jiedu yukui formula showed significant dose-effect relationship. CONCLUSIONS: Huazhuo jiedu yukui formula can improve disease activity status of UC rats and reduce colon mucosal damage, and the effects of medium-dose and high-dose Huazhuo jiedu yukui formula were better than mesalamine, which may be related to reducing inflammatory reaction and inhibiting COX-2 expression in colon tissue.
期刊: 2017年第28卷第10期
编辑: 孙春霞,?霍永利,郭喜军,张晓艳,马小顺
AUTHORS: SUN Chunxia,HUO Yongli,GUO Xijun,ZHANG Xiaoyan,MA Xiaoshun
关键字: 化浊解毒愈溃方;溃疡性结肠炎;黏膜损伤;炎症因子;环氧合酶2;大鼠
KEYWORDS: Huazhuo jiedu yukui formula; Ulcerative colitis; Mucosal damage; Inflammatory cytokines; Cyclooxygenase-2; Rats
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