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单剂量顺铂对大鼠肾间质纤维化指标的影响
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篇名: 单剂量顺铂对大鼠肾间质纤维化指标的影响
TITLE:
摘要: 目的:探讨单剂量顺铂对大鼠肾间质纤维化指标的影响。方法:将72只SD大鼠随机分为正常组和顺铂组,每组36只。正常组与顺铂组大鼠于实验第1天分别单次腹腔注射等体积生理盐水、顺铂5 mg/kg,于实验第8、14、30、50、60、90天分别处死6只大鼠,测定血清尿素氮(BUN)和肌酐(Cr)水平,检测肾小管间质损伤程度、肾小管间质纤维化相对面积和肾组织中α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原(Col Ⅰ)和转化生长因子β1(TGF-β1)表达情况。结果:与正常组比较,顺铂组大鼠各时间点血清BUN和Cr水平、肾小管间质损伤指数、肾小管间质纤维化相对面积和肾组织中α-SMA、Col Ⅰ、TGF-β1表达水平均明显升高(P<0.05或P<0.01)。在顺铂组中,在第8~90天内,大鼠血清BUN水平无明显变化,血清Cr水平、肾小管间质损伤指数、肾小管间质纤维化相对面积和肾组织中α-SMA、Col Ⅰ、TGF-β1表达水平均呈先升高后降低趋势。结论:临床剂量的顺铂单次给药可诱导大鼠肾间质纤维化,其机制可能与其肾组织中TGF-β1表达有关。
ABSTRACT: OBJECTIVE: To discuss the effects of single dose of cisplatin on renal interstitial fibrosis indicators in rats dynamically. METHODS: 72 SD rats were randomly divided into normal group and cisplatin group, with 36 rats in each group. Normal group and cisplatin group were given equal volume of normal saline and cisplatin 5 mg/kg intraperitoneally on the first day, respectively. Each 6 rats were sacrificed on 8th, 14th, 30th, 50th, 60th, 90th day. The serum levels of blood urea nitrogen (BUN) and creatinine (Cr) were determined, and the degree of renal tubulointerstitial injury and relative area of renal tubulointerstitial fibrosis were evaluated. The expression of α-smooth muscle actin (α-SMA), type I collagen (Col Ⅰ) and transforming growth factor β1 (TGF-β1) were determined in renal tissue. RESULTS: Compared with normal group, the serum levels of BUN and Cr, renal tubulointerstitial injury indexes, relative area of renal tubulointerstitial fibrosis, and the expression of α-SMA, Col Ⅰ and TGF-β1 in renal tissue were increased significantly (P<0.05 or P<0.01). In cisplatin group, within the 8th-90th days, serum level of BUN in rats had no significant change; serum level of Cr, renal tubulointerstitial injury indexes, renal tubulointerstitial fibrosis, the expression of α-SMA, Col Ⅰ and TGF-β1 in renal tissue increased first and then decreased. CONCLUSIONS: A single dose of clinical dose of cisplatin can induce renal interstitial fibrosis in rats, and its mechanism may be related to the expression of TGF-β1 in renal tissue.
期刊: 2018年第29卷第3期
编辑: 陆斯斯,钟小斌,杨玉芳,邹小琴,梁雪艳,蔡果珍
AUTHORS: LU Sisi,ZHONG Xiaobin,YANG Yufang,ZOU Xiaoqin,LIANG Xueyan,CAI Guozhen
关键字: 顺铂;肾间质纤维化;α-平滑肌肌动蛋白;Ⅰ型胶原蛋白;转化生长因子-β1;大鼠
KEYWORDS: Cisplatin; Renal interstitial fibrosis; α-SMA; Col Ⅰ; TGF-β1; Rat
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