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HPLC法同时测定桑菊感冒颗粒中5种有效成分的含量
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篇名: HPLC法同时测定桑菊感冒颗粒中5种有效成分的含量
TITLE:
摘要: 目的:建立同时测定桑菊感冒颗粒中绿原酸、芦丁、连翘苷、槲皮素和甘草酸含量的方法。方法:采用高效液相色谱法。色谱柱为WondasilTM-C18,流动相为乙腈-0.4%磷酸溶液(梯度洗脱),流速为0.8 mL/min,柱温为30 ℃,检测波长为254 nm,进样量为5 μL。结果:绿原酸、芦丁、连翘苷、槲皮素和甘草酸的检测进样量线性范围分别为0.014~0.560 μg(r=0.999 2)、0.022~0.880 μg(r=0.999 6)、0.010~0.400 μg(r=0.999 9)、0.016~0.640 μg(r=0.999 9)、0.011~0.440 μg(r=0.999 1);定量限分别为0.752、0.647、0.483、0.372、0.242 mg/L,检测限分别为0.253、0.185、0.157、0.162、0.098 mg/L;精密度、稳定性、重复性试验的RSD均小于2.0%;加样回收率分别为 97.4%~100.9%(RSD=1.4%,n=6)、95.7%~99.0%(RSD=1.1%,n=6)、95.1%~98.1%(RSD=1.3%,n=6)、96.4%~99.8%(RSD=1.4%,n=6)、97.8%~100.4%(RSD=1.0%,n=6)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于桑菊感冒颗粒中绿原酸、芦丁、连翘苷、槲皮素和甘草酸含量的同时测定。
ABSTRACT: OBJECTIVE: To establish a method for the content determination of chlorogenic acid, rutin, phillyrin, quercetin and glycyrrhizic acid in Sangju ganmao granules. METHODS: HPLC method was adopted. The determination was performed on WondasilTM-C18 column with mobile phase consisted of acetonitrile-0.4% phosphoric acid solution (gradient elution) at the flow rate of 0.8 mL/min. The column temperature was set at 30 ℃, and the detection wavelength was set at 254 nm. The sample size was 5 μL. RESULTS: The linear range of chlorogenic acid, rutin, phillyrin, quercetin and glycyrrhizic acid were 0.014-0.560 μg(r=0.999 2),0.022-0.880 μg(r=0.999 6),0.010-0.400 μg(r=0.999 9),0.016-0.640 μg(r=0.999 9),0.011-0.440 μg(r=0.999 1), respectively. The limits of quantitation were 0.752, 0.647, 0.483, 0.372, 0.242 mg/L; the limits of detection were 0.253, 0.185, 0.157, 0.162, 0.098 mg/L. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The average recoveries were 97.4%-100.9%(RSD=1.4%,n=6), 95.7%-99.0%(RSD=1.1%,n=6), 95.1%-98.1%(RSD=1.3%,n=6), 96.4%-99.8%(RSD=1.4%,n=6), 97.8%-100.4%(RSD=1.0%,n=6), respectively. CONCLUSIONS: The method is simple, precise, stable and reproducible, and can be used for simultaneous determination of chlorogenic acid, rutin, phillyrin, quercetin and glycyrrhizic acid in Sangju ganmao granules.
期刊: 2018年第29卷第12期
编辑: 崔佰吉,张宏梅,朱鹤云,冯波
AUTHORS: CUI Baiji,ZHANG Hongmei,ZHU Heyun,FENG Bo
关键字: 桑菊感冒颗粒;高效液相色谱法;绿原酸;芦丁;连翘苷;槲皮素;甘草酸;含量测定
KEYWORDS: Sangju gamao granules; HPLC; Chlorogenic acid; Rutin; Phillyrin; Quercetin; Glycyrrhizic acid; Content determination
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