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UPLC法同时测定野菊花中8种活性成分的含量
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篇名: UPLC法同时测定野菊花中8种活性成分的含量
TITLE:
摘要: 目的:建立同时测定野菊花中新绿原酸、绿原酸、隐绿原酸、咖啡酸、异绿原酸C、木犀草苷、蒙花苷、芹菜素8种活性成分含量的方法。方法:采用超高效液相色谱法。色谱柱为Waters ACQUITY UPLC BEH C18,流动相为0.1%磷酸溶液-乙腈(梯度洗脱),流速为0.3 mL/min,检测波长为335 nm,柱温为35 ℃,进样量为1 μL,样品室温为8 ℃。结果:新绿原酸、绿原酸、隐绿原酸、咖啡酸、异绿原酸C、木犀草苷、蒙花苷和芹菜素进样量检测线性范围分别为0.140~1.680、2.211~26.532、0.178~2.136、0.056~0.672、0.460~5.520、1.260~15.120、2.725~32.700、0.120~0.144 ng(r>0.999 6);检测限分别为0.02、0.02、0.02、0.01、0.01、0.01、0.04、0.01 ng,定量限分别为0.06、0.07、0.07、0.03、0.04、0.04、0.13、0.02 ng;精密度、稳定性(10 h)、重复性试验的RSD均<2%(n=6),平均加样回收率为97.05%~102.04%(RSD为1.03%~1.65%,n=9)。结论:建立的方法分析速度快、灵敏度和分辨率较高、重复性好、结果准确,可用于野菊花中8种活性成分含量的同时测定。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of 8 active components in Dendranthema indicum, such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid C, luteolin, monososide and apigenin. METHODS: UPLC method was adopted. The determination was performed on Waters ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1% phosphoric acid solution-acetonitrile (gradient elution) at the flow rate of 0.3 mL/min. The detection wavelength was set at 335 nm and column temperature was maintained at 35 ℃. The sample size was 1 μL and sample room temperature was 8 ℃. RESULTS: The linear range of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid C, luteolin, monososide and apigenin were 0.140-1.680, 2.211-26.532, 0.178-2.136, 0.056-0.672, 0.460-5.520, 1.260-15.120, 2.725-32.700, 0.120-0.144 ng (r>0.999 6), respectively. The limits of detection were 0.02, 0.02, 0.02, 0.01, 0.01, 0.01, 0.04, 0.01 ng, and the limits of quantitation were 0.06, 0.07, 0.07, 0.03, 0.04, 0.04, 0.13, 0.02 ng, respectively. RSDs of precision, stability (10 h) and reproducibility tests were all lower than 2% (n=6). Average recoveries were 97.05%-102.04%(RSD=1.03%-1.65%, n=9). CONCLUSIONS: The established method has high analysis speed, high sensitivity, high resolution, good repeatability and accurate results. It can be used for simultaneous determination of 8 active components in D. indicum.
期刊: 2018年第29卷第15期
编辑: 范帅帅,田伟,王相,高乐,田宇柔,牛丽颖
AUTHORS: FAN Shuaishuai,TIAN Wei,WANG Xiang,GAO Le,TIAN Yurou,NIU Liying
关键字: 野菊花;超高效液相色谱法;活性成分;含量测定
KEYWORDS: Dendranthema indicum; UPLC; Active components; Content determination
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