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重庆道地药材独活的生药学研究
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篇名: 重庆道地药材独活的生药学研究
TITLE:
摘要:

目的:建立鉴别重庆道地药材独活的生药学方法。方法:从原植物形态、性状特征、显微特征(横切面、粉末)、薄层色谱

(TLC)方面鉴别独活。结果:独活外形上根圆锥状,褐色,多分枝,具特异香气;主根横切面与侧根横切面显微特征无区别,初生木

质部为二原型,次生韧皮部较次生木质部发达,次生韧皮部中有丰富的油室。维管组织中含有大量薄壁组织细胞。TLC色谱中,

供试品与蛇床子素和二氢欧山芹醇当归酸酯以[石油醚(60~90 ℃)-乙酸乙酯(7 ∶3,V/V)为展开剂,紫外光灯下检视]对照品色谱

相应位置上显相同颜色的荧光斑点。结论:该方法操作简单、准确度高,可作为独活生药学鉴别的依据。

ABSTRACT:

OBJECTIVE:To establish a method for the identification of Chongqing local medicinal material Heracleum hemsleyanum.

METHODS:The original plant morphology,morphological characteristics,microscopic characteristics (transverse section

and powder),were identified. RESULTS:H. hemsleyanum had cone shape root,brown,many-branched with special fragrance;the

microscopic characteristics of taproot a transverse showed no differences with lateral root transverse section,primary xylem was diarch,

secondary phloem was more developed than secondary xylem,secondary phloem was rich in the oil chamber. Vascular tissues

contained a large number of parenchyma cells. In TLC chromatrogram,the test sample showed the same color fluorescent spots withthe

substance reference of osthole and columbianadin in the corresponding locations. CONCLUSIONS:The established method is

simple with high accuracy,and can be used as the basis for identification of H. hemsleyanum.

期刊: 2015年第26卷第36期
编辑: 封海霞,石燕红,陈绍成
AUTHORS: FENG Hai-xia,SHI Yan-hong,CHEN Shao-cheng
关键字: 独活;生药学;原植物形态;性状特征;显微特征;薄层色谱
KEYWORDS: Heracleum hemsleyanum;Pharmacognosy;Original plant morphology;Morphological characteristic;Microscopic
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