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利咽合剂的质量标准研究
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篇名: 利咽合剂的质量标准研究
TITLE:
摘要:

目的:建立利咽合剂的质量标准。方法:采用薄层色谱(TLC)法对制剂中的玄参、西青果、甘草进行鉴别;采用高效液相

色谱法测定制剂中绿原酸的含量。色谱柱为Agilent Eclipse Plus C18,流动相为甲醇-0.2%磷酸(18 ∶82,V/V),流速为1.0 ml/min,检

测波长为327 nm,柱温为30 ℃,进样量为20 μl。结果:玄参、西青果、甘草的TLC图斑点清晰,分离度好,阴性对照无干扰。绿原

酸检测进样量线性范围为0.16~1.6 μg(r=0.999 9);精密度、稳定性、重复性的RSD<1.0%,加样回收率为96.9%~101.4%,RSD

为1.17%(n=9)。结论:该方法操作简单、重复性好,可用于利咽合剂的质量控制。

ABSTRACT:

OBJECTIVE:To establish the quality standard of Liyan mixture. METHODS:TLC was conducted to identify the

Scrophularia ningpoensis,Terminalia chebula and Glycyrrhiza uralensis;HPLC was conducted to determine the content of chlorogenic

acid. The column was Agilent Eclipse Plus C18 with mobile phase of methanol-0.2% phosphoric acid(18 ∶ 82,V/V)at flow

rate of 1.0 ml/min,the detection wavelength was 327 nm,column temperature was 30 ℃ and the injection volume was 20 μl. RESULTS:

TLC showed S. ningpoensis,T. chebula and G. uralensis had clear spots and good separation. There was no interference

for negative control. The linear range of chlorogenic acid was 0.16-1.6 μg(r=0.999 9);RSDs of precision,stability and reproducibility

tests were lower than 1.0%,recovery was 96.9%-101.4%(RSD=1.17%,n=9). CONCLUSIONS:The method is simple

and reproducible,and can be used for the quality control of Liyan mixture.

期刊: 2015年第26卷第36期
编辑: 景霞,徐进,孙芳,湛雯
AUTHORS: JING Xia,XU Jin,SUN Fang,ZHAN Wen
关键字: 利咽合剂;质量标准;玄参;西青果;甘草;薄层色谱法;绿原酸;高效液相色谱法
KEYWORDS: Liyan mixture;Quality standard;Scrophularia ningpoensis;Terminalia chebula;Glycyrrhiza uralensis;TLC;
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