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乌芪舒筋通络片中乌头碱限量检测方法研究
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篇名: 乌芪舒筋通络片中乌头碱限量检测方法研究
TITLE:
摘要:

目的:建立测定乌芪舒筋通络片中乌头碱限量的方法。方法:采用薄层色谱(TLC)法对制剂中的乌头碱进行鉴定。薄层

板为硅胶G薄层板,展开剂为甲苯-乙酸乙酯-三氯甲烷-丙酮-氨水(20 ∶ 18 ∶ 3 ∶ 6 ∶ 1,V/V/V/V/V),显色剂为稀碘化铋钾试液和亚硝酸

钠乙醇试液。经耐用性和检测限考察,并优选薄层板、温度和湿度。结果:乌头碱的TLC图斑点清晰且阴性对照无干扰。本试验

耐用性良好;检测限为0.9 μg;可用薄层板为Merck HPTLC预制板、硅胶G薄层板和自制的以羧甲基纤维素钠为黏合剂的硅胶G

薄层板;温度为5~16 ℃,湿度为32%~72%。结论:本方法操作简便、重复性良好,可用于乌芪舒筋通络片中乌头碱限量的测定。

ABSTRACT:

OBJECTIVE:To establish a method for the limit detection of aconitine in Wuqi shujin tongluo tablet. METHODS:

TLC was conducted to identify the aconitine;TLC plate was silica gel G plate,developing solvent was toluene-ethyl acetate-chloroform-

acetone-ammonia(20 ∶18 ∶3 ∶6 ∶1,V/V/V/V/V),chromogenic agent was bismuth potassium iodide test solution and sodium nitrite

ethanol test solution;and durability investigation and detection limit detection were used to optimize the TLC plate,temperature

and humidity. RESULTS:TLC showed the aconitine had clear spots and negative control without interference. The durability

was good;detection limit was 0.9 μ g;available TLC plate was Merck HPTLC prefabricated plate,silica gel G TLC plate

setf-made silica gel G TLC plate with adhesive of sodium carboxymethycellulose;temperature was 5-16 ℃ and humidity was

32%-72%. CONCLUSIONS:The method is simple and reproducibility,and can be used for the limit detection of aconitine in

Wuqi shujin tongluo tablet.

期刊: 2015年第26卷第36期
编辑: 陈震尧,陈金英,姚伟生
AUTHORS: CHEN Zhen-yao,CHEN Jin-ying,YAO Wei-sheng
关键字: 乌芪舒筋通络片;乌头碱;限量;薄层色谱法
KEYWORDS: Wuqi shujin tongluo tablet;Aconitine;Limit;TLC
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