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HPLC法测定银黄清火胶囊中绿原酸的含量
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篇名: HPLC法测定银黄清火胶囊中绿原酸的含量
TITLE:
摘要:

目的:建立测定银黄清火胶囊中绿原酸含量的方法。方法:采用高效液相色谱法。色谱柱为Acclaim120? C18,流动相为

乙腈-0.4%磷酸(12 ∶88,V/V),流速为1 ml/min,检测波长为327 nm,柱温为25 ℃,进样量为10 μl。结果:绿原酸检测进样量线性范

围为56~224 μg;精密度、稳定性、重复性的RSD<3.0%;加样回收率为96.91%~104.76%,RSD为1.46%(n=9)。结论:该方法

操作简便、重复性好,可用于银黄清火胶囊的质量控制。

ABSTRACT:

OBJECTIVE:To establish a method to determine the content of chlorogenic acid in Yinhuang qinghuo capsule.

METHODS:HPLC was performed on the column of Acclaim120? C18 with mobile phase of acetonitrile -0.4% phosphoric acid(12 ∶

88,V/V)at flow rate of 1 ml/min,the detection wavelength was 327 nm,column temperature was 25 ℃,and volume injection was

10 μl. RESULTS:The linear range of chlorogenic acid was 56-224 μg;RSDs of precision,stability and reproduciblity tests were

lower than 3.0%,recovery was 96.91%-104.76%(RSD=1.46%,n=9). CONCLUSIONS:The method is simple and reproducible,

and can be used for the quality control of Yinhuang qinghuo capsule.

期刊: 2015年第26卷第36期
编辑: 解立刚,陈赫军,何世学,何芳
AUTHORS: XIE Li-gang,CHEN He-jun,HE Shi-xue,HE Fang
关键字: 银黄清火胶囊;绿原酸;高效液相色谱法;含量测定
KEYWORDS: Yinhuang qinghuo capsule;Chlorogenic acid;HPLC;Content determination
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