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HPLC法测定白灵片中芍药苷的含量及不确定度评价
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篇名: HPLC法测定白灵片中芍药苷的含量及不确定度评价
TITLE:
摘要:

目的:建立测定白灵片中芍药苷含量的方法,并对测量结果进行不确定度评价。方法:采用高效液相色谱法。色谱柱为

Platisil C18,流动相为水-乙腈(87 ∶13,V/V),流速为1 ml/min,检测波长为230 nm,柱温为25 ℃,进样量为10 μl。对测定过程和步骤

进行量化分析,评价其不确定度。结果:芍药苷检测进样量线性范围为0.100 1~2.502 8 μg;精密度、稳定性、重复性试验的RSD<

2.0%,加样回收率为98.7%~100.9%,RSD为0.86%(n=6)。对照品称量、稀释过程和方法准确度对合成不确定度的贡献较大。

结论:该方法可用于白灵片中芍药苷的含量测定,不确定度评价提供了此方法的可信度。

ABSTRACT:

OBJECTIVE:To establish a method to determinate the content of paeoniflorin in Bailing tablet,and to evaluate the

uncertainty of results. METHODS:HPLC was performed on the column of Platisil C18 with mobile phase of water - acetonitrile(87 ∶

13,V/V)at a flow rate of 1 ml/min,the detection wavelength was 230 nm,the column temperature was 25 ℃ and the injection

volume was 10 μ l. Quantitative analysis was conducted for the processes and procedures and the uncertainty was evaluated.

RESULTS:The linear range of paeoniflorin was 0.100 1-2.502 8 μg;RSDs of precision,stability and reproducibility tests were

lower than 2.0%,recovery rate was 98.7%-100.9%(RSD=0.86%,n=6). Reference substance amount,elution produce and the

accuracy of method had great effect on the uncertainty. CONCLUSIONS:The established method can be used for the content

determination of paeoniflorin in Bailing tablet,and the uncertainty evaluation provides reliability for the method.

期刊: 2015年第26卷第36期
编辑: 栗建明,侯惠婵,顾利红
AUTHORS: LI Jian-ming,HOU Hui-chan,GU Li-hong
关键字: 白灵片;芍药苷;含量测定;不确定度
KEYWORDS: Bailing tablet;Paeoniflorin;Content determination;Uncertainty
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