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艳山姜提取物对急性胃溃疡模型小鼠的保护作用研究
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篇名: 艳山姜提取物对急性胃溃疡模型小鼠的保护作用研究
TITLE: Study on the Protective Effect of Alpinia zerumbet Extract on Acute Gastric Ulcer Model Mice
摘要: 目的:探讨艳山姜提取物对急性胃溃疡模型小鼠的保护作用。方法:取小鼠48只,随机分为空白组、模型组、阳性组(三九胃泰颗粒,20mg/kg)和艳山姜提取物高、中、低剂量组(2.34、1.17、0.59g/kg,按生药量计),每组8只,每天灌胃生理盐水或相应药物1次,连续给药7d后,除空白组外的其余组小鼠均一次性灌胃无水乙醇0.1mL/10g,以建立急性胃溃疡模型。另取小鼠48只,同法分组,连续给药15d,并于给药第10天起对除空白组外的其余组小鼠连续6d灌胃阿司匹林(20mg/kg),以建立急性胃溃疡模型。在乙醇诱导模型实验中,观察各组小鼠胃溃疡形成情况并测定胃溃疡指数;采用苏木精-伊红染色法观察胃组织病理变化;采用酶联免疫吸附法(ELISA)检测血清中胃泌素(GAS)和胃组织中国足球协会超级联赛氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)的水平。在阿司匹林诱导模型实验中,同法观察并测定各组小鼠胃溃疡形成情况、胃溃疡指数;采用ELISA法检测血清中GAS、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)和胃组织中前列腺素E(2PGE2)、环氧合酶2(COX-2)的水平。结果:与空白组比较,模型组小鼠胃组织均出现了明显的溃疡病变;血清中GAS、TNF-α、IL-1β和胃组织中MDA、NO水平均显著升高,胃组织中SOD、PGE2、COX-2水平均显著降低(P<0.05或P<0.01)。与模型组比较,给药组小鼠的胃组织病变程度有不同程度减轻;胃溃疡指数有不同程度降低,血清和胃组织中上述指标水平均有不同程度改善,大部分指标差异有统计学意义(P<0.05或P<0.01)。结论:艳山姜提取物对无水乙醇和阿司匹林诱导的小鼠急性胃溃疡均有保护作用;其机制可能是通过抑制氧化应激和炎症反应,从而减轻胃黏膜损伤。
ABSTRACT: OBJECTIVE:To explore the protective effect of Alpinia zerumbet extract on acute gastric ulcer model mice. METHODS:Totally 48 mice were collected and randomly divided into blank group ,model group ,positive group (Sanjiu weitai granules,20 mg/kg),A. zerumbet extract high-dose ,medium-dose and low-dose groups (2.34,1.17,0.59 g/kg,by crude drug ), with 8 mice in each group. They were given normal saline or relevant medicine intragastrically ,once a day ,for consecutive 7 d. Then,except for blank group ,other groups were given disposable intragastric administration of absolute ethanol 0.1 mL/10 g to establish acute gastric ulcer model. Another 48 mice were collected and grouped with same method ,and then given relevant medicine for consecutive 15 d. From the 10th day of administration ,except for blank group ,other groups were given intragastric administration of aspirin (20 mg/kg) for consecutive 6 d to establish acute gastric ulcer model. In ethanol induction model experiment,the formation of gastric ulcer and the index of gastric ulcer were observed in each group ;HE staining was used to observe the pathological changes of gastric tissue ;the levels of gastrin (GAS)in serum ,and the levels of SOD ,MDA and NO in gastric tissue were determined by ELISA. In the aspirin induced model experiment ,the formation of gastric ulcer and the index of gastric ulcer were observed and measured by the same method ;the levels of GAS ,TNF-α,IL-1β in serum,and the levels of PGE2 and COX- 2 in gastric tissue were determined by ELISA. RESULTS :Compared with model group ,obvious ulcer lesions were found in gastric tissue of rats in model group ;the levels of GAS ,TNF-α,IL-1β in serum,and MDA ,NO in gastric tissue were increased significantly , while the levels of SOD , PGE2, (No.QZYY-2019- COX-2 in gastric tissue were decreased si gnificantly(P<0.05 063). Compared with model group ,the degree of gastric lesions in administration groups was alleviated to some extent;gastric ulcer index was decreased to some extent , mail:997845460@qq.com while the levels of the above indexes in serum and gastric tissue were improved in varying degrees , with statistical 制。E-mail:672863283@qq.com significance in most indexes (P<0.05 or P<0.01). CONCLUSIONS:A. zerumbet extract exerts its protective effect on absolute ethanol and a spirin-induced acute gastric ulcer model mice,the mechanism of which may be relieving gastric mucosal injury through inhibiting oxidative stress and inflammatory response.
期刊: 2020年第31卷第18期
编辑: 魏晴,梁珊珊,熊瑞,魏娜,薛娟
AUTHORS: WEI Qing,LIANG Shanshan ,XIONG Rui,WEI Na,XUE Juan
关键字: 艳山姜;提取物;急性胃溃疡;无水乙醇;阿司匹林;保护作用;小鼠
KEYWORDS: Alpinia zerumbet ;Extract;Acute gastric ulcer ;Absolute ethanol ;Aspirin;Protective effect ;Mice
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