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二苯乙烯苷对人乳腺癌T-47D细胞增殖及其雌激素受体表达的影响
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篇名: 二苯乙烯苷对人乳腺癌T-47D细胞增殖及其雌激素受体表达的影响
TITLE: Effects of Stilbene Glucoside on the Proliferation and Estrogen Receptor Expression of Human Breast Cancer T-47D Cells
摘要: 目的:研究二苯乙烯苷(TSG)对人乳腺癌T-47D细胞增殖及其雌激素受体(ER)表达的影响,探讨其雌激素样作用及可能机制。方法:以ER阳性人乳腺癌T-47细胞为对象,以β-雌二醇(β-E2,1×10-8mol/L)为阳性对照,采用CKK-8法检测不同浓度TSG(1×10-8、1×10-7、1×10-6、1×10-5、1×10-4mol/L)作用24、48、72h后的细胞增殖情况,并计算增殖率;分别采用Westernblotting法和逆转录-聚合酶链式反应法检测经低、中、高浓度TSG(1×10-8、1×10-6、1×10-4mol/L)作用48h后细胞中ER-α、ER-β蛋白及其mRNA的表达情况。结果:经不同浓度TSG作用24、48、72h后,各给药组各时间点(除β-E2组48h外)的细胞增殖率均较空白组显著升高,且TSG1×10-7、1×10-6、1×10-5mol/L组显著高于β-E2组(P<0.05或P<0.01)。经低、中、高浓度TSG作用48h后,各给药组细胞中ER-α、ER-β蛋白及其mRNA的表达量均较空白组显著升高(P<0.05或P<0.01);且TSG低浓度组ER-β蛋白的表达量,各浓度组细胞中ER-αmRNA以及低、高浓度组ER-βmRNA的表达量均显著高于β-E2组(P<0.05或P<0.01)。结论:TSG可诱导T-47D细胞的体外增殖,并可通过促进ER-α、ER-β蛋白及其mRNA的表达来发挥雌激素样作用;这种作用在一定浓度下强于β-E2。
ABSTRACT: OBJECTIVE:To study the effects of stilbene glucoside (TSG)on the proliferation and estrogen receptor (ER)of human breast cancer T- 47D cells ,and to explore its estrogen-like effect and potential mechanism. METHODS :Taking ER positive human breast cancer T- 47D cells as subjects ,using β-estradiol(β-E2,1×10-8 mol/L)as positive control ,CCK-8 assay was used to detect the cell proliferation after treated with different concentrations of TSG (1×10-8,1×10-7,1×10-6,1×10-5,1×10-4 mol/L)for 24,48,72 h;the cell proliferation rate was calculated. Western blotting assay and RT-PCR methods were adopted to detect the protein and mRNA expression of ER-α and ER-β in cells after treated with low,medium and high concentrations of TSG (1×10-8, 1×10-6,1×10-4 mol/L)for 48 h. RESULTS :After treated with different concentrations of TSG for 24,48,72 h,the cell proliferation rate of each administration group at each time point (except for β-E2 group at 48 h)increased significantly ,compared with blank group ;those of TSG groups (1×10-5,1×10-6,1×10-7 mol/L)were significantly higher than β-E2 group(P<0.05 or P<0.01). After treated with low ,medium and high concentrations of TSG for 48 h,protein and mRNA expression of ER-α and ER-β in cells were increased significantly,compared with blank group (P<0.05 or P<0.01);protein expression of ER-β in TSG low concentration group ,mRNA expression of ER-α in TSG groups as well as mRNA expression of ER-β in TSG low and high concentration groups were significantly higher than β-E2 group(P<0.05 or P<0.01). CONCLUSIONS :TSG can induce the in vitro proliferation of T- 47D cells and exert estrogen-like effects by promoting protein and mRNA expression of ER-α and ER-β, which is stronger than that of β-E2 at a certain concentration.
期刊: 2020年第31卷第19期
编辑: 朱璨,王嫣,李尧锋,付云燕,唐文超,杨长福,王和生
AUTHORS: ZHU Can,WANG Yan,LI Yaofeng ,FU Yunyan ,TANG Wenchao ,YANG Changfu ,WANG Hesheng
关键字: 二苯乙烯苷;植物雌激素;人乳腺癌T-47D细胞;细胞增殖;雌激素受体
KEYWORDS: Stilbene glucoside ;Phytoestrogen;Human breast cancer T- 47D cells ;Cell proliferation ;Estrogen receptor
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