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甘草次酸修饰多西紫杉醇磁性纳米粒的制备与表征
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篇名: 甘草次酸修饰多西紫杉醇磁性纳米粒的制备与表征
TITLE: Preparation and Characterization of Glycyrrhetinic Acid-modified Docetaxel Magnetic Nanoparticles
摘要: 目的:制备甘草次酸修饰多西紫杉醇磁性纳米粒(GA-DTX-NGO/IONP-NPs),并对其理化性质进行评价。方法:以磁性纳米氧化石墨烯(NGO/IONP)作为抗肿瘤药物载体,多西紫杉醇(DTX)为模型药物,甘草次酸(GA)为靶头分子。采用水热法合成NGO/IONP、酰胺化反应合成GA修饰的壳聚糖(GA-CS)后,采用傅里叶红外光谱法、差示扫描量热法及振动样品磁测量法等对两者进行表征。采用离子凝胶化法制备GA-DTX-NGO/IONP-NPs;采用透射电镜、纳米粒度分析仪等对其微观形态、粒径及Zeta电位进行观察和测定;采用超滤离心法测定其包封率和载药量;通过观察有无外加磁场时的状态考察其磁性;结合808nm激光对其进行光热转换试验。结果:成功合成了NGO/IONP和GA-CS,且NGO/IONP呈现超顺磁性。GA-DTX-NGO/IONP-NPs在透射电镜下呈圆球状,粒径为(262.8±4.23)nm,Zeta电位为(13.6±1.51)mV,包封率为(94.29±0.50)%,载药量为(17.12±0.12)%。GA-DTX-NGO/IONP-NPs的外观呈黑色,分散均匀;其在外加磁场下磁性纳米粒可定向移动,显示出良好的磁定向性。在808nm激光照射下,GA-DTX-NGO/IONP-NPs具有良好的光热转换效应,且呈浓度和时间依赖趋势。结论:本研究成功制备了一种磁性纳米载药系统GA-DTX-NGO/IONP-NPs,可为肿瘤的磁热-化疗联合治疗提供一定的理论依据。
ABSTRACT: OBJECTIVE:To pre pare Glycyrrhetinic acid-modified docetaxel magnetic nanoparticles (GA-DTX-NGO/IONP- NPs),and to evaluate its physicochemical properties. METHODS :Magnetic nano graphene oxide (NGO/IONP)was chosen as the anti-tumor drug carrier ,docetaxel(DTX)as the model drug and glycyrrhetinic acid (GA)as the target molecule. Firstly ,NGO/ IONP was synthesized by hydrothermal method and GA-CS was synthesized by amidation reaction. Fourier IR spectrometer ,DSC and vibration sample magnetic measuring instrument were used to characterize NGO/IONP and GA-CS. GA-DTX-NGO/IONP-NPs Huperzine A in the nicotinic acetylcholine receptor alleviates Aβ -induced 1-42 treatment of Alzheimer ’s disease and vascular dementia :a neurotoxicity via downregulation of p 38 and JNK MAPK meta-analysis[J]. Evid Based Complement Alternat Med , signaling pathways[J]. Neurochem Int ,2018. DOI :10. 2014. DOI :10.1155/2014/363985. 1016/j.neuint.2018.09.005. were prepared by the ion gelation method. TEM and particle size analyzer were used to observe and determine the morphology , particle size and Zeta potential of GA-DTX-NGO/IONP-NPs ;the ultrafiltration-centrifugation method was used to determine encapsulation efficiency and drug loading amount ;the magnetic properties were investigated by investigating the state with or without external magnetic field ;the photothermal conversion test was carried out with laser irradiation of 808 nm. RESULTS :NGO/ IONP and GA-CS were successfully synthesized ,and NGO/IONP exhibited superparamagnetism characteristics. GA-DTX-NGO/ IONP-NPs were spherical under TEM ,the particle size was (262.8±4.23)nm and the Zeta potential was (13.6±1.51)mV. The encapsulation rate and drug loading amount were (94.29±0.50)% and(17.12±0.12)%,respectively. GA-DTX-NGO/IONP-NPs were black in appearance and evenly dispersed. Under the external magnetic field ,the magnetic nanoparticles could move directionally,showing good magnetic properties. GA-DTX-NGO/IONP-NPs showed a good concentration- and time-dependent photothermal conversion effect under 808 nm laser irradiation. CONCLUSIONS :GA-DTX-NGO/IONP-NPs are successfully prepared. This study could provide some theoretical basis for the combined treatment of magnetic heating-chemotherapy for liver tumors.
期刊: 2020年第31卷第19期
编辑: 王莎莎,陈家琦,王华华,黄胜楠,贾永艳,祝侠丽
AUTHORS: WANG Shasha ,CHEN Jiaqi,WANG Huahua ,HUANG Shengnan ,JIA Yongyan ,ZHU Xiali
关键字: 磁性纳米氧化石墨烯;甘草次酸;多西紫杉醇;磁性纳米粒
KEYWORDS: Magnetic nano graphene oxide ;Glycyrrhetinic acid ;Docetaxel;Magnetic nanoparticles
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