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基于Met/PI3K/Akt信号通路研究五味子甲素对人鼻咽癌细胞HONE-1增殖、迁移和侵袭的影响
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篇名: 基于Met/PI3K/Akt信号通路研究五味子甲素对人鼻咽癌细胞HONE-1增殖、迁移和侵袭的影响
TITLE: Study on the Effects of Deoxyschizandrin on the Proliferation ,Migration and Invasion of Human Nasopharyngeal Carcinoma Cell HONE- 1 via Met/PI 3K/Akt Signaling Pathway
摘要: 目的:研究五味子甲素对人鼻咽癌细胞HONE-1增殖、迁移和侵袭的影响及其可能机制。方法:以HONE-1细胞为研究模型,使用不同浓度[0(空白对照)、10、20、40μmol/L]的五味子甲素处理后,分别采用CCK-8试验、划痕试验和Transwell小室试验检测细胞的增殖、迁移和侵袭能力变化;通过计算机分子对接分析五味子甲素与酪氨酸蛋白激酶(Met)蛋白的结合能力;采用Westernblotting法检测细胞中磷酸化酪氨酸蛋白激酶(p-Met)、磷酸化磷脂酰肌醇-3-激酶(p-PI3K)、磷酸化蛋白激酶B(p-Akt)、B淋巴细胞瘤2(Bcl-2)和N-钙黏蛋白(N-cadherin)的相对表达量。结果:与空白对照比较,10、20、40μmol/L五味子甲素处理后细胞的增殖、迁移和侵袭能力均显著减弱(P<0.05);分子对接结果显示,五味子甲素能与Met蛋白的活性口袋稳定结合;Westernblot-ting试验结果显示,与空白对照比较,10、20、40μmol/L五味子甲素处理后细胞中p-Met、p-PI3K、p-Akt、Bcl-2和N-cadherin蛋白的相对表达量均显著降低(P<0.05)。结论:五味子甲素可通过抑制Met/PI3K/Akt信号通路的活化来抑制HONE-1细胞的增殖、迁移和侵袭。
ABSTRACT: OBJECTIVE:To study the effects and potential mechani sm of deoxyschizandrin on the proliferation ,migration and invasion of nasopharyngeal carcinoma cell HONE- 1. METHODS :HONE-1 cell was set as cell model ,while CCK- 8 test,wound healing assay and Transwell chamber test were used to detect the proliferation ,migration and invasion ability changes of HONE- 1 cells after treatment with different concentrations [ 0(blank control ),10,20,40 μmol/L] of deoxyschizandrin. Computer molecular docking was performed to analyze the binding ability between deoxyschizandrin and Met protein. Western blotting assay was used to detect the relative protein expressions of p-Met ,p-PI3K,p-Akt,Bcl-2 and N-cadherin in cells. RESULTS :Compared with blank control ,the proliferation ,migration and invasion ability of cells after treated with 10,20,40 μmol/L deoxyschizandrin were all decreased significantly (P<0.05). Results of molecular docking revealed that deoxyschizandrin could stably bind with the activity pocket of Met protein. Results of Western blotting assay demonstrated that compared with blank control ,10,20,40 μmol/L deoxyschizandrin all decreased the relative protein expressions of p-Met ,p-PI3K,p-Akt,Bcl-2 and N-cadherin in cells significantly(P<0.05). CONCLUSIONS :Deoxyschizandrin can inhibit the proliferation ,migration and invasion of HONE- 1 cell via inhibiting the activation of Met/PI 3K/Akt signaling pathway.
期刊: 2020年第31卷第19期
编辑: 陈腾祥,梁黎,曾智锐,雷珊,王婧雅,孙远梅,兰金芝,薛燕
AUTHORS: CHEN Tengxiang,LIANG Li,ZENG Zhirui,LEI Shan,WANG Jingya,SUN Yuanmei,LAN Jinzhi,XUE Yan
关键字: 五味子甲素;增殖;迁移;侵袭;酪氨酸蛋白激酶;磷脂酰肌醇-3-激酸;蛋白激酶B;人鼻咽癌细胞HONE-1
KEYWORDS: Deoxyschizandrin;Proliferation;Migration;
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