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基于灰色关联分析的藤茶不同极性部位在小鼠体内抗炎作用的谱-效关系研究
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篇名: 基于灰色关联分析的藤茶不同极性部位在小鼠体内抗炎作用的谱-效关系研究
TITLE: Study on Spectrum-effect Relationship of Anti-inflammatory Effect of Different Polar Parts of Ampelopsis grossedentata in Mice Based on Grey Relational Analysis
摘要: 目的:研究藤茶不同极性部位的高效液相色谱(HPLC)指纹图谱与其体内抗炎作用的谱-效关系。方法:取藤茶药材,以70%乙醇回流提取,再分别以石油醚、氯仿、乙酸乙酯、水饱和正丁醇萃取,或直接以水煎煮,浓缩后获得藤茶不同极性部位。建立二甲苯致小鼠耳肿胀炎症模型,以地塞米松为阳性对照,分别考察藤茶不同极性部位的抗炎活性。采用HPLC法建立藤茶不同极性部位的指纹图谱,色谱柱为Poroshell120EC-C18,流动相为乙腈-0.1%磷酸水溶液(梯度洗脱),流速为1mL/min,柱温为25℃,检测波长为365nm,进样量为5μL。运用灰色关联度法分析藤茶不同极性部位HPLC指纹图谱共有峰与其抗炎药效之间的谱-效关系,计算关联系数、关联度并进行排序。结果:小鼠抗炎实验结果显示,藤茶70%乙醇提取总部位、乙酸乙酯萃取部位、水提取部位的抗炎活性最明显(小鼠耳肿胀抑制率分别为54.07%、30.54%、30.45%)。藤茶不同极性部位HPLC指纹图谱中共确定了5个共有峰。谱-效关系分析结果显示,5个共有峰的关联度均大于0.6;其中,抗炎效果较强的是3号峰和2号峰(二氢杨梅素),两者的关联度均大于0.8。结论:藤茶对二甲苯致小鼠耳肿胀的抗炎作用是多组分协同作用的结果;3号峰未知成分和二氢杨梅素可能是藤茶抗炎作用的主要活性成分。
ABSTRACT: OBJECTIVE:To study the spectrum-effect relationship of HPLC finger print of different polar parts of Ampelopsis grossedentata with its in vivo anti-inflammatory effect. METHODS :A. grossedentata was reflux extracted with 70% ethanol,then extracted with petroleum ether ,chloroform,ethyl acetate and water saturated n-butanol;or it was directly decocted with water and then concentrated to obtain different polar parts. The xylene-induced mice ear swelling model was established ;using dexamethasone as positive control ,anti-inflammatory activity of different polar parts of A. grossedentata was investigated. Fingerprints of different polar parts of A. grossedentata were established by HPLC. The determination was performed on Poroshell 120 EC-C18 column with mobile phase consisted of acetonitrile- 0.1% phosphoric acid solution (gradient elution )at the flow rate of 1 mL/min. The column temperature was 25 ℃. The detection wavelength was set at 365 nm,and sample size was 5 μL. The grey ralational analysis method was used to analyze the spectrum-effect relationship of HPLC fingerprint common peaks of different polar parts of A. grossedentata with its anti-inflammatory effect. The correlation coefficient and correlation degree were calculated and ranked. RESULTS:Anti-inflammatory experiment showed that the anti-inflammatory effects of 70% ethanol extraction part ,ethyl acetate extraction part and water extraction part were the most significant (inhibitory rates of ear swelling were 54.07%,30.54%, 30.45%). Five common peaks were determined in HPLC fingerprints of different polar parts from A. grossedentata . The spectrum-effect analysis results showed that the correlation of5 common peaks were higher than 0.6;among them ,peak 3 and peak 2 (dihydromyricetin) had the strongest anti- inflammatory effect ,and their correlation degrees were both mail:123745789@qq.com greater than 0.8. CONCLUSIONS : The anti-inflammatory effect of A. grossedentata on xylene-induced ear swelling in mice is the result of multi-comp onent synergy ; unknown substance of peak 3 and dihydromyricetin may be the main active components of A. grossedentata .
期刊: 2020年第31卷第19期
编辑: 刘雯,刘云,刘进宝,郭海姣,郑丽贞,周丽园,钟燕妮,覃洁萍
AUTHORS: LIU Wen,LIU Yun,LIU Jinbao ,GUO Haijiao ,ZHENG Lizhen ,ZHOU Liyuan ,ZHONG Yanni,QIN Jieping
关键字: 藤茶;指纹图谱;抗炎活性;谱-效关系;灰色关联分析
KEYWORDS: Ampelopsis grossedentata;Fingerprint;Anti-inflammatory activity ;Spectrum-effect relationship ;Grey relational
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