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青翘和老翘的HPLC指纹图谱比较及聚类分析、主成分分析
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篇名: 青翘和老翘的HPLC指纹图谱比较及聚类分析、主成分分析
TITLE: Comparison of HPLC Fingerprint ,Cluster Analysis and Principle Component Analysis of Green Forsythia sus- pensa and Grown F. suspensa
摘要: 目的:建立青翘和老翘药材的指纹图谱并比较,同时进行聚类分析和主成分分析。方法:采用高效液相色谱法。色谱柱为HypersilC18,流动相为乙腈-0.1%甲酸水溶液(梯度洗脱),检测波长为235nm,柱温为25℃,流速为1.0mL/min,进样量为10μL。以连翘苷为参照,绘制8批青翘(Q1~Q8)和6批老翘(L1~L6)药材的HPLC指纹图谱;采用《中药色谱指纹图谱相似度评价系统(2012版)》进行相似度评价,确定共有峰;采用SPSS23.0App进行聚类分析和主成分分析。结果:青翘和老翘药材共有19个共有峰,指认了其中6个,分别为连翘酯苷A、芦丁、松脂素-β-D-葡萄糖苷、连翘苷、槲皮素、连翘脂素;青翘与老翘药材的相似度为0.351~0.767。聚类分析结果显示,青翘和老翘药材样品可聚为4类,其中L1~L6聚为一类、Q1聚为一类、Q2~Q6聚为一类、Q7~Q8聚为一类。主成分分析结果显示,前3个主成分的累积方差贡献率为83.14%,L1~L6分布较近,Q2~Q6分布较近,Q7~Q8分布较近,Q1分布较为独立,与聚类分析结果一致。结论:青翘和老翘的指纹图谱共有峰经相似度评价、聚类分析及主成分分析均有明显差异,所建HPLC指纹图谱可用于综合评价和比较青翘和老翘药材的质量。
ABSTRACT: OBJECTIVE:To e stablish and compare HPLC fingerprints of green Forsythia suspensa and grown F. suspensa ,and to conduct cluster analysis and principle component analysis. METHODS :HPLC method was adopted. The determination was performed on Hypersil C 18 column with mobile phase consisted of acetonitrile- 0.1% formic acid (gradient elution ). The detection wavelength was 235 nm and column temperature was 25 ℃ with the flow rate of 1.0 mL/min. The sample size was 10 μL. HPLC fingerprints of 8 batches of green F. suspensa (Q1-Q8)and 6 batches of grown F. suspensa (L1-L6)were drawn ,with phillyrin as reference;the similarity evaluation was conducted by using Similarity Evaluation System of TCM Chromatographic Fingerprint (2012 edition),and common peak was confirmed. Cluster analysis and principal component analysis were carried out with SPSS 23.0 software. RESULTS :There were 19 common peaks for green F. suspensa and grown F. suspensa ,among which 6 peaks were identified,i.e. forsythoside A ,rutin,pinoresinol-β-D-glucoside,phillyrin,quercetin and phillygenin ;the similarities of HPLC fingerprints from green F. suspensa and grown F. suspensa were 0.351-0.767;results of cluster analysis showed that green F. suspensa and grown F. suspensa were classified into 4 categories,among which L 1-L6 were clustered into one category ,Q1 was clustered into one category ,Q2-Q6 were clustered into one category ;Q7-Q8 were clustered into one category. The results of principal component analysis showed that the cumulative variance contribution rate of the first three principal components was 83.14%, L1-L6 distribution was close ,Q2-Q6 distribution was close ,Q7-Q8 distribution was close ,and Q 1 distribution was independent , which was consistent with the results of cluster analysis. CONCLUSIONS :There were significant differences in the common peaks of fingerprint of green F. suspensa and grown F. suspensa of similarity eraluation ,cluster analysis and principle component analysis,the established HPLC fingerprint can be used for comprehensive evaluation and quality comparison of green F. suspensa and grown F. suspensa .
期刊: 2021年第32卷第06期
编辑: 王越欣,苗雨露,王梅,李宁,武英茹,张文智,冯敏,倪艳
AUTHORS: WANG Yuexin ,MIAO Yulu,WANG Mei,LI Ning,WU Yingru ,ZHANG Wenzhi ,FENG Min,NI Yan
关键字: 青翘;老翘;高效液相色谱法;指纹图谱;相似度分析;聚类分析;主成分分析
KEYWORDS: Green Forsythia suspensa ;Grown Forsythia
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