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青稞多糖对糖尿病模型小鼠的降血糖作用及机制研究
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篇名: 青稞多糖对糖尿病模型小鼠的降血糖作用及机制研究
TITLE: Study on Hypoglycemic Effect of Hordeum vulgare Polysaccharide on Diabetes Mellitus Model Mice and Its Mechanism
摘要: 目的:研究青稞多糖(HVP)对糖尿病模型小鼠的降血糖作用及机制。方法:对小鼠腹腔注射链脲佐菌素(120mg/kg)以复制糖尿病模型。将造模成功的小鼠随机分为模型组、二甲双胍组(阳性对照,200mg/kg)和HVP高、中、低剂量组(300、150、75mg/kg),另设空白对照组,每组10只。各给药组灌胃相应药物,空白对照组和模型组小鼠灌胃等量水,每天1次,连续30天。给药10、20、30天时测定小鼠的空腹血糖(FBG);末次FBG测定后,小鼠腹腔注射10%葡萄糖溶液(2g/kg),于注射后30、120min时测定糖耐受量(GTT)曲线下面积;测定小鼠血清中胰岛素水平和肝组织中国足球协会超级联赛氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的含量;采用苏木精-伊红染色法观察小鼠胰腺组织病理学变化。结果:与空白对照组比较,模型组小鼠FBG、GTT曲线下面积和肝组织中MDA含量均显著升高或增加(P<0.01);血清中胰岛素水平和肝组织中SOD、GSH-Px含量均显著降低(P<0.01);胰腺组织损伤严重,形态不完整,有明显的空泡。与模型组比较,HVP高剂量组小鼠FBG、GTT曲线下面积和肝组织中MDA含量均显著降低或减少(P<0.05或P<0.01),血清中胰岛素含量和肝组织中SOD、GSH-Px含量均显著升高(P<0.05或P<0.01),HVP低、中剂量组上述指标部分改善,差异具有统计学意义(P<0.05或P<0.01);胰腺组织损伤有所减轻,形态较为完整,胰岛细胞排列紧密,空泡减少。结论:HVP可降低糖尿病模型小鼠的血糖水平,升高胰岛素水平并减轻胰腺组织损伤;该作用机制可能与其抗氧化作用有关。
ABSTRACT: OBJECTIVE:To study the h ypoglycemic effect of Hordeum vulgare polysaccharide(HVP)on diabetes mellitus model mice and its mechanism. METHODS :The mice were given intraperitoneal injection of streptozotocin (120 mg/kg)to induce diabetes mellitus model. After modeling ,the mice were randomly divided into model group ,metformin group (positive control , 200 mg/kg),HVP high-dose ,medium-dose and low-dose groups (300,150 and 75 mg/kg),with 10 mice in each group. Blank control group was established additionally. Administration groups were given relevant medicine intragastrically ;blank control group and model group were given constant volume of water intragastrically ,once a day ,for consecutive 30 days. The levels of fasting blood glucose (FBG)were determined after 10,20,30 days of administration. After last FBG test ,mice were intraperitoneally injected with 10% glucose solution (2 g/kg),then the area under the glucose tolerance (GTT)curve was measured at 30 and 120 min after injection. The serum levels of insulin ,the content of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px), malondialdehyde(MDA)in liver tissue were detected. Pancreatic morphology of mice were detected by HE staining. RESULTS : Compared with blank control group ,the FBG ,area under GTT curve and MDA contnet in liver tissue were increased significantly in model group (P<0.01),while serum levels of insulin ,SOD and GSH-Px contents in liver tissue were decreased significantly (P<0.01);the pancreatic tissue was seriously damaged with incomplete morphology and obvious vacuoles. Compared with model group,FBG,area under GTT curve ,MDA content in liver Δ 基金项目:国家自然科学基金资助项目(No.81473179) tissue were decreased significantly in HVP high-dose group *主管药师 。研究方向 :临床药学 。电话:0510-85351490。E- mail:Wangshengya_helen@126.com (P<0.05 or P<0.01),serum content of insulin ,the content # 通信编辑 :副主任药师 。研究方向 :医院药学 。电话:0510- of SOD and GSH-Px in liver tissue were increased 85351490。E-mail:3103221944@qq.com significantly(P<0.05 or P<0.01);above indexes of HVP 威尼斯在线平台 2021年第32卷第7期 China Pharmacy 2021Vol. 32 No. 7 ·807· low-dose and medium-dose groups were improved partially ,with statistical significance (P<0.05 or P<0.01);the pancreatic tissue damage was alleviated ,the morphology was relatively complete ,the islet cells were closely arranged ,and the vacuoles were reduced. CONLUSIONS :HVP can reduce the level of blood glucose in diabetic model mice ,increase insulin level and relieve pancreatic injury. The mechanism may be related to its antioxidation.
期刊: 2021年第32卷第07期
编辑: 王生亚,薛洁,徐乃玉,张真庆,吕栋
AUTHORS: WANG Shengya ,XUE Jie,XU Naiyu,ZHANG Zhenqing ,LYU Dong
关键字: 青稞多糖;糖尿病;抗氧化;小鼠
KEYWORDS: Hordeum vulgare polysaccharide;Diabetes mellitus ;Antioixidation;Mice
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