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蒲公英质量标准提升及不同产地药用蒲公英质量评价
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篇名: 蒲公英质量标准提升及不同产地药用蒲公英质量评价
TITLE: Quality Standard Improve of Taraxaci Herba and Quality Evaluation of T. officinale from Different Origins
摘要: 目的:提升蒲公英药材质量标准,并评价不同产地药用蒲公英药材的质量。方法:在2020年版《中国药典》(一部)“蒲公英”项下规定的基础上,增加水溶性浸出物(热浸法)、醇溶性浸出物(热浸法)、总黄酮、绿原酸、咖啡酸、菊苣酸和异绿原酸A的含量测定方法,同时以8个产地42批药用蒲公英药材为对象建立高效液相色谱(HPLC)指纹图谱,并基于上述结果进行主成分分析。结果:42批药用蒲公英药材的醇溶性浸出物含量为15.30%~30.40%,水溶性浸出物含量为27.59%~38.96%。总黄酮(紫外-可见分光光度法)和绿原酸、咖啡酸、菊苣酸、异绿原酸A(HPLC法)的质量浓度分别在0.016~0.096、0.003~0.196、0.004~0.117、0.025~1.578、0.002~0.152mg/mL范围内线性关系良好(R2均大于0.9990);精密度、稳定性、重复性试验的RSD均小于2.00%(n=6);平均加样回收率为98.97%~103.53%,RSD为1.19%~1.58%。42批药用蒲公英药材中总黄酮、绿原酸、咖啡酸、菊苣酸、异绿原酸A的含量分别为0.734%~3.700%、0.004%~0.123%、0.006%~0.087%、0.073%~1.499%、0.005%~0.109%。42批药用蒲公英样品的HPLC指纹图谱共有峰相对保留时间的RSD为0~0.94%,相对峰面积的RSD为0~125.57%,其中39批样品相似度大于0.900。主成分分析结果显示,陕西产药用蒲公英药材的综合质量较优,河北产药材次之。结论:拟定蒲公英药材中醇溶性浸出物、水溶性浸出物、总黄酮、绿原酸、咖啡酸、菊苣酸、异绿原酸A的含量分别不得低于17.0%、27.0%、1.383%、0.024%、0.021%、0.450%、0.021%。陕西产药用蒲公英药材除咖啡酸含量相对较低外,其余各项指标均较优;河北产药材的咖啡酸含量高于陕西产药材,其他各项指标略低于陕西产药材;其余产地的药用蒲公英药材综合质量相对较差,且同一产地不同批次间的药材质量不稳定。
ABSTRACT: OBJECTIVE:To improve the quality standard of Taraxaci Herba ,and to evaluate the quality of T. officinale from different origins. METHODS :Based on the provisions of the 2020 edition of Chinese Pharmacopoeia (part Ⅰ)under the item “Taraxaci Herba ”,the method of content determination was added for the detection of water-soluble extracts (hot extraction method)and alcohol-soluble extracts (hot extraction method ),total flavonoids ,chlorogenic acid ,caffeic acid ,cichoric acid and isochlorogenic acid A. HPLC fingerprint was established by using 42 batches of T. officinale from 8 producing areas as object ,and principal component analysis was performed on the basis of above results. RESULTS :The contents of alcohol-soluble extracts in 42 batches of T. officinale were 15.30%-30.40%,and those of water-soluble extracts were 27.59%-38.96%. The concentration of total flavonoids(UV spectrophotometry ),chlorogenic acid ,caffeic acid ,cichoric acid and isochlorogenic acid A (HPLC method )were 0.016-0.096,0.003-0.196,0.004-0.117,0.025-1.578,0.002-0.152 mg/mL,respectively (all R2>0.999);RSDs of precision , stability and repeatability tests were all lower than 2.00%(n=6);average sample recovery were 98.97%-103.53%,and RSDs were 1.19%-1.58%. The contents of total flavonoids ,chlorogenic acid ,caffeic acid ,cichoric acid and isochlorogenic acid A were 0.734% -3.700% ,0.004% -0.123% ,0.006% - 0.087% ,0.073% -1.499% ,0.005% -0.109% respectively in 42 batches of T. officinale. For 42 batches of T. officinale samples in HPLC fingerprint ,RSDs of the relative retention time of the common peak were 0-0.94%,and RSDs of the relative peak area were 0-125.57%. Among them ,the similarity of 39 batches of samples was all higher than 0.900. Results of principal component analysis showed that the quality of T. officinale from Shaanxi province was better,followed by medicinal materials from Hebei province. CONCLUSIONS :Tentatively,the contents of alcohol-soluble extract,water-soluble extract ,total flavonoids ,chlorogenic acid ,caffeic acid ,cichoric acid and isochlorogenic acid A shall not be less than 17.0%,27.0%,1.383%,0.024%,0.021%,0.450%,0.021% for Taraxaci Herba. In addition to the low content of caffeic acid in T. officinale from Shaanxi province ,the other indexes were better ;the content of caffeic acid in T. officinale from Hebei province was higher than that from Shaanxi province ,and other indicators were slightly lower than that from Shaanxi province. The quality of comprehensive evaluation of T. officinale from other origins was relatively poor ,and the quality of different batches of medicinal materials from the same origin was unstable.
期刊: 2021年第32卷第07期
编辑: 惠西珂,李超,谷巍,巢建国,王凯,韩怡,王玉卓,王圆圆,张松保
AUTHORS: HUI Xike, LI Chao,GU Wei,CHAO Jianguo,WANG Kai,HAN Yi,WANG Yuzhuo,WANG Yuanyuan,ZHANG Songbao
关键字: 蒲公英;质量标准;质量评价;高效液相色谱法;指纹图谱;主成分分析;药用蒲公英
KEYWORDS: Taraxaci Herba ;Quality standard ;Quality evaluation ;HPLC;Fingerprint;Principal component analysis ;
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